GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury

Chromatin regulators (CRs) are essential upstream regulatory factors of epigenetic modification. The role CRs in the pathogenesis renal ischemia-reperfusion injury (IRI) remains unclear. We analyzed a bioinformatic analysis on differentially expressed chromatin regulator genes IRI patients using data from public domains. hub identified were used to develop risk prediction model for IRI, and their expressions also validated Western blot, qRT-PCR, immunohistochemistry murine model. examined relationships between infiltrating immune cells network explore drugs that target relevant downstream microRNAs. results machine learning methods showed five (DUSP1, GADD45A, GADD45B, GADD45G, HSPA1A) upregulated with key roles cell cycle, p38 MAPK signaling pathway, p53 FoxO NF-κB pathway. Two network, GADD45A GADD45B (growth arrest DNA damage-inducible protein 45 alpha beta), chosen They all good performance testing validation cohorts. Mice significantly expression within kidneys compared sham-operated mice. correlations plasmacytoid dendritic (pDCs) enrichment pathway based weighted gene co-expression (WGCNA) method. Candidate include beta-escin, sertraline, primaquine, pimozide, azacyclonol. dysregulation is related infiltration pDCs, may have therapeutic potential IRI.